Design of Large-Scale Reporter Construct Arrays for Dynamic, Live Cell Systems Biology.

ABSTRACT: Dynamic systems biology aims to identify the molecular mechanisms governing cell fate decisions through the analysis of living cells. Large scale molecular information from living cells can be obtained from reporter constructs that provide activities for either individual transcription factors or multiple factors binding to the full promoter following CRISPR/Cas9 directed insertion of luciferase. In this report, we investigated the design criteria to obtain reporters that are specific and responsive to transcription factor (TF) binding and the integration of TF binding activity with genetic reporter activity. The design of TF reporters was investigated for the impact of consensus binding site spacing sequence and off-target binding on the reporter sensitivity using a library of 25 SMAD3 ac

SUBMITTER: Decker JT 

PROVIDER: S-EPMC11293491 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature