Single-cell analysis reveals transcriptional dynamics in healthy primary parathyroid tissue.

ABSTRACT: Studies on human parathyroids are generally limited to hyperfunctioning glands owing to the difficulty in obtaining normal human tissue. We therefore obtained non-human primate (NHP) parathyroids to provide a suitable alternative for sequencing that would bear a close semblance to human organs. Single-cell RNA expression analysis of parathyroids from four healthy adult M. mulatta reveals a continuous trajectory of epithelial cell states. Pseudotime analysis based on transcriptomic signatures suggests a progression from GCM2 ^hi progenitors to mature parathyroid hormone (PTH)-expressing epithelial cells with increasing core mitochondrial transcript abundance along pseudotime. We sequenced, as a comparator, four histologically characterized hyperfunctioning hum

SUBMITTER: Venkat A 

PROVIDER: S-EPMC11293540 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature