Mapping the Single-Cell Differentiation Landscape of Osteosarcoma.

ABSTRACT:

Purpose

The genetic intratumoral heterogeneity observed in human osteosarcomas poses challenges for drug development and the study of cell fate, plasticity, and differentiation, which are processes linked to tumor grade, cell metastasis, and survival.

Experimental design

To pinpoint errors in osteosarcoma differentiation, we transcriptionally profiled 31,527 cells from a tissue-engineered model that directs mesenchymal stem cells toward adipogenic and osteoblastic fates. Incorporating preexisting chondrocyte data, we applied trajectory analysis and non-negative matrix factorization to generate the first human mesenchymal differentiation atlas.

Results

This "roadmap" served as a reference to delineate the cellular composition of morphologically complex osteosarcoma tum

SUBMITTER: Truong DD 

PROVIDER: S-EPMC11293971 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature