Functional determination of site-mutations in rdxA involved in metronidazole resistance of Helicobacter pylori.

ABSTRACT:

Background

Metronidazole (MTZ) is among the first-line drugs against the human gastric pathogen Helicobacter pylori (H. pylori). MTZ is used as a prodrug that is activated by an oxygen-insensitive enzyme NADPH nitroreductase (RdxA). Loss-of-function mutations in rdxA make H. pylori MTZ resistant; however, experimental proof is lacking.

Methods

We collected 139 gastric biopsy samples from patients suspected of H. pylori infection in Shanghai, and amplified Hp-specific rdxA gene from 134 samples. All these rdxA genes were sequenced and phylogenetically compared. The effect of mutations on RdxA function was measured by expressing them in Escherichia coli DH5α by using the MTZ sensitivity test.

Results

In tota

SUBMITTER: Huang J 

PROVIDER: S-EPMC11294100 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature