Engineering a GPCR-based yeast biosensor for a highly sensitive melatonin detection from fermented beverages.

ABSTRACT: Melatonin is a multifunctional molecule with diverse biological roles that holds great value as a health-promoting bioactive molecule in any food product and yeast's ability to produce it has been extensively demonstrated in the last decade. However, its quantification presents costly analytical challenges due to the usual low concentrations found as the result of yeast metabolism. This study addresses these analytical challenges by optimizing a yeast biosensor based on G protein-coupled receptors (GPCR) for melatonin detection and quantitation. Strategic genetic modifications were employed to significantly enhance its sensitivity and fluorescent signal output, making it suitable for detection of yeast-produced melatonin. The optimized biosensor demonstrated significantly improved sensitiv

SUBMITTER: Bisquert R 

PROVIDER: S-EPMC11294354 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature