Z-DNA formation in promoters conserved between human and mouse are associated with increased transcription reinitiation rates.

ABSTRACT: A long-standing question concerns the role of Z-DNA in transcription. Here we use a deep learning approach DeepZ that predicts Z-flipons based on DNA sequence, structural properties of nucleotides and omics data. We examined Z-flipons that are conserved between human and mouse genomes after generating whole-genome Z-flipon maps and then validated them by orthogonal approaches based on high resolution chemical mapping of Z-DNA and the transformer algorithm Z-DNABERT. For human and mouse, we revealed similar pattern of transcription factors, chromatin remodelers, and histone marks associated with conserved Z-flipons. We found significant enrichment of Z-flipons in alternative and bidirectional promoters associated with neurogenesis genes. We show that conserved Z-flipons are associated with

SUBMITTER: Beknazarov N 

PROVIDER: S-EPMC11294368 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature