Directed differentiation of human embryonic stem cells into parathyroid cells and establishment of parathyroid organoids.

ABSTRACT: Differentiation of human embryonic stem cells (hESCs) into human embryonic stem cells-derived parathyroid-like cells (hESC-PT) has clinical significance in providing new therapies for congenital and acquired parathyroid insufficiency conditions. However, a highly reproducible, well-documented method for parathyroid differentiation remains unavailable. By imitating the natural process of parathyroid embryonic development, we proposed a new hypothesis about the in vitro differentiation of parathyroid-like cells. Transcriptome, differentiation marker protein detection and parathyroid hormone (PTH) secretion assays were performed after the completion of differentiation. To optimize the differentiation protocol and further improve the differentiation rate, we designed glial cells missing transc

SUBMITTER: Wang G 

PROVIDER: S-EPMC11294423 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature