Subzero project: comparing trace element profiles of enriched mitochondria fractions from frozen and fresh liver tissue.

ABSTRACT: From organs to subcellular organelles, trace element (TE) homeostasis is fundamental for many physiological processes. While often overlooked in early stages, manifested TE disbalance can have severe health consequences, particularly in the context of aging or pathological conditions. Monitoring TE concentrations at the mitochondrial level could identify organelle-specific imbalances, contributing to targeted diagnostics and a healthier aging process. However, mitochondria isolation from frozen tissue is challenging, as it poses the risk of TE losses from the organelles due to cryodamage, but would significantly ease routine laboratory work. To address this, a novel method to isolate an enriched mitochondria fraction (EMF) from frozen tissue was adapted from already established protocols.

SUBMITTER: Heinze T 

PROVIDER: S-EPMC11294433 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature