Spatially targeted chemokine exocytosis guides transmigration at lymphatic endothelial multicellular junctions.

ABSTRACT: Migrating cells preferentially breach and integrate epithelial and endothelial monolayers at multicellular vertices. These sites are amenable to forces produced by the migrating cell and subsequent opening of the junctions. However, the cues that guide migrating cells to these entry portals, and eventually drive the transmigration process, are poorly understood. Here, we show that lymphatic endothelium multicellular junctions are the preferred sites of dendritic cell transmigration in both primary cell co-cultures and in mouse dermal explants. Dendritic cell guidance to multicellular junctions was dependent on the dendritic cell receptor CCR7, whose ligand, lymphatic endothelial chemokine CCL21, was exocytosed at multicellular junctions. Characterization of lymphatic endothelial secretory

SUBMITTER: Liaqat I 

PROVIDER: S-EPMC11294460 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature