The Impact of Calcium Depletion on Proliferation of Chlorella sorokiniana Strain DSCG150.

ABSTRACT: This study analyzed the effects of Ca²⁺ metal ions among culture medium components on the Chlorella sorokiniana strain DSCG150 strain cell growth. The C. sorokiniana strain DSCG150 grew based on a multiple fission cell cycle and growth became stagnant in the absence of metal ions in the medium, particularly Ca²⁺. Flow cytometry and confocal microscopic image analysis results showed that in the absence of Ca²⁺, cell growth became stagnant as the cells accumulated into four autospores and could not transform into daughter cells. Genetic analysis showed that the absence of Ca²⁺ caused upregulation of calmodulin (calA) and cell division control protein 2 (CDC2_1) genes, and downregulation of origin of replication complex s

SUBMITTER: Kang S 

PROVIDER: S-EPMC11294638 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature