Transcriptional and methylation outcomes of didehydro-cortistatin A use in HIV-1-infected CD4⁺ T cells.

ABSTRACT: Ongoing viral transcription from the reservoir of HIV-1 infected long-lived memory CD4⁺ T cells presents a barrier to cure and associates with poorer health outcomes for people living with HIV, including chronic immune activation and inflammation. We previously reported that didehydro-cortistatin A (dCA), an HIV-1 Tat inhibitor, blocks HIV-1 transcription. Here, we examine the impact of dCA on host immune CD4⁺ T-cell transcriptional and epigenetic states. We performed a comprehensive analysis of genome-wide transcriptomic and DNA methylation profiles upon long-term dCA treatment of primary human memory CD4⁺ T cells. dCA prompted specific transcriptional and DNA methylation changes in cell cycle, histone, interferon-response, and T-cell lineage transcription

SUBMITTER: Mori LP 

PROVIDER: S-EPMC11294679 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature