PANAMA-enabled high-sensitivity dual nanoflow LC-MS metabolomics and proteomics analysis.

ABSTRACT: High-sensitivity nanoflow liquid chromatography (nLC) is seldom employed in untargeted metabolomics because current sample preparation techniques are inefficient at preventing nanocapillary column performance degradation. Here, we describe an nLC-based tandem mass spectrometry workflow that enables seamless joint analysis and integration of metabolomics (including lipidomics) and proteomics from the same samples without instrument duplication. This workflow is based on a robust solid-phase micro-extraction step for routine sample cleanup and bioactive molecule enrichment. Our method, termed proteomic and nanoflow metabolomic analysis (PANAMA), improves compound resolution and detection sensitivity without compromising the depth of coverage as compared with existing widely used analytical p

SUBMITTER: Lin W 

PROVIDER: S-EPMC11294829 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature