Phage Display Panning on Silica: Optimization of Elution Conditions for Selection of Strong Binders.

ABSTRACT: Phage display panning is a powerful tool to select strong peptide binders to a given target, and when applied to inorganic materials (e.g., silica) as a target, it provides information on binding events and molecular recognition at the peptide-mineral interface. The panning process has limitations with the phage chemical elution being affected by bias toward positively charged binders, resulting in the potential loss of information on binder diversity; the presence of fast growing phages with an intrinsic growth advantage; and the presence of false positives from target unrelated peptides. To overcome some of these limitations, we developed a panning approach based on the sequential use of different eluents (Gly-HCl, pH-2.2; MgCl₂, pH-6.1; and TEA, pH-11.0), or pH conditions (Gl

SUBMITTER: Thota V 

PROVIDER: S-EPMC11295192 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature