Gadolinium-Based NMR Spin Relaxation Measurements of Near-Surface Electrostatic Potentials of Biomolecules.

ABSTRACT: NMR spectroscopy is an important tool for the measurement of the electrostatic properties of biomolecules. To this point, paramagnetic relaxation enhancements (PREs) of ¹H nuclei arising from nitroxide cosolutes in biomolecular solutions have been used to measure effective near-surface electrostatic potentials (ϕ_ENS) of proteins and nucleic acids. Here, we present a gadolinium (Gd)-based NMR method, exploiting Gd chelates with different net charges, for measuring ϕ_ENS values and demonstrate its utility through applications to a number of biomolecular systems. The use of Gd-based cosolutes offers several advantages over nitroxides for ϕ_ENS measurements. First, unlike nitroxide compounds, Gd chelates enable electrostatic potential measurements on o

SUBMITTER: Yu B 

PROVIDER: S-EPMC11295196 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature