N⁶-methyladenosine-modified SRPK1 promotes aerobic glycolysis of lung adenocarcinoma via PKM splicing.

ABSTRACT:

Background

The RNA N⁶-methyladenosine (m⁶A) modification has become an essential hotspot in epigenetic modulation. Serine-arginine protein kinase 1 (SRPK1) is associated with the pathogenesis of various cancers. However, the m⁶A modification of SRPK1 and its association with the mechanism of in lung adenocarcinoma (LUAD) remains unclear.

Methods

Western blotting and polymerase chain reaction (PCR) analyses were carried out to identify gene and protein expression. m⁶A epitranscriptomic microarray was utilized to the assess m⁶A profile. Loss and gain-of-function assays were carried out elucidate the impact of METTL3 and SRPK1 on LUAD glycolysis and tumorigenesis. RNA immunoprecipitation (RIP), m⁶A RNA immunoprec

SUBMITTER: Wang A 

PROVIDER: S-EPMC11295518 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature