Microglial heterogeneity in the ischemic stroke mouse brain of both sexes.

ABSTRACT:

Background

Ischemic stroke elicits a complex and sustained immune response in the brain. Immunomodulatory treatments have long held promise for improving stroke outcomes, yet none have succeeded in the clinical setting. This lack of success is largely due to our incomplete understanding of how immune cells respond to stroke. The objective of the current study was to dissect the effect of permanent stroke on microglia, the resident immune cells within the brain parenchyma.

Methods

A permanent middle cerebral artery occlusion (pMCAO) model was used to induce ischemic stroke in young male and female mice. Microglia were sorted from fluorescence reporter mice after pMCAO or sham surgery and then subjected to single-cell RNA sequencing analysis. Various methods, including flow cy

SUBMITTER: Del Aguila A 

PROVIDER: S-EPMC11295600 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature