Vascular restoration through local delivery of angiogenic factors stimulates bone regeneration in critical size defects.

ABSTRACT: Critical size bone defects represent a significant challenge worldwide, often leading to persistent pain and physical disability that profoundly impact patients' quality of life and mental well-being. To address the intricate and complex repair processes involved in these defects, we performed single-cell RNA sequencing and revealed notable shifts in cellular populations within regenerative tissue. Specifically, we observed a decrease in progenitor lineage cells and endothelial cells, coupled with an increase in fibrotic lineage cells and pro-inflammatory cells within regenerative tissue. Furthermore, our analysis of differentially expressed genes and associated signaling pathway at the single-cell level highlighted impaired angiogenesis as a central pathway in critical size bone defects,

SUBMITTER: Fang L 

PROVIDER: S-EPMC11295624 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature