Metabolic disruption impairs ribosomal protein levels, resulting in enhanced aminoglycoside tolerance.

ABSTRACT: Aminoglycoside antibiotics target ribosomes and are effective against a wide range of bacteria. Here, we demonstrated that knockout strains related to energy metabolism in Escherichia coli showed increased tolerance to aminoglycosides during the mid-exponential growth phase. Contrary to expectations, these mutations did not reduce the proton motive force or aminoglycoside uptake, as there were no significant changes in metabolic indicators or intracellular gentamicin levels between wild-type and mutant strains. Our comprehensive proteomics analysis unveiled a noteworthy upregulation of proteins linked to the tricarboxylic acid (TCA) cycle in the mutant strains during the mid-exponential growth phase, suggesting that these strains compensate for the perturbation in their energy metab

SUBMITTER: Shiraliyev R 

PROVIDER: S-EPMC11296704 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature