Alternative proteoforms and proteoform-dependent assemblies in humans and plants.

ABSTRACT: The variability of proteins at the sequence level creates an enormous potential for proteome complexity. Exploring the depths and limits of this complexity is an ongoing goal in biology. Here, we systematically survey human and plant high-throughput bottom-up native proteomics data for protein truncation variants, where substantial regions of the full-length protein are missing from an observed protein product. In humans, Arabidopsis, and the green alga Chlamydomonas, approximately one percent of observed proteins show a short form, which we can assign by comparison to RNA isoforms as either likely deriving from transcript-directed processes or limited proteolysis. While some detected protein fragments align with known splice forms and protein cleavage events, multiple examples are previou

SUBMITTER: McWhite CD 

PROVIDER: S-EPMC11297038 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature