PET imaging of colon cancer CD73 expression using cysteine site-specific ⁸⁹Zr-labeled anti-CD73 antibody.

ABSTRACT: CD73 is a cell-surface ectoenzyme that hydrolyzes the conversion of extracellular adenosine monophosphate to adenosine, which in turn can promote resistance to immune checkpoint blockade therapy. Immune response may therefore be improved by targeting tumor CD73, and this possibility underlines the need to non-invasively assess tumor CD73 level. In this study, we developed a cysteine site-specific ⁸⁹Zr-labeled anti-CD73 (⁸⁹Zr-CD73) IgG immuno-PET technique that can image tumor CD73 expression in living bodies. Anti-CD73 IgG was reduced with tris(2-carboxyethyl)phosphine, underwent sulfohydryl moiety-specific conjugation with deferoxamine-maleimide, and was radiolabeled with ⁸⁹Zr. CT26 mouse colon cancer cells, CT26/CD73 cells engineered to constitutively ove

SUBMITTER: Jung KH 

PROVIDER: S-EPMC11297922 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature