ArcS from Thermococcus kodakarensis transfers L-lysine to preQ₀ nucleoside derivatives as minimum substrate RNAs.

ABSTRACT: Archaeosine (G⁺) is an archaea-specific tRNA modification synthesized via multiple steps. In the first step, archaeosine tRNA guanine transglucosylase (ArcTGT) exchanges the G15 base in tRNA with 7-cyano-7-deazaguanine (preQ₀). In Euryarchaea, preQ₀15 in tRNA is further modified by archaeosine synthase (ArcS). Thermococcus kodakarensis ArcS catalyzes a lysine-transfer reaction to produce preQ₀-lysine (preQ₀-Lys) as an intermediate. The resulting preQ₀-Lys15 in tRNA is converted to G⁺15 by a radical S-adenosyl-L-methionine enzyme for archaeosine formation (RaSEA), which forms a complex with ArcS. Here, we focus on the substrate tRNA recognition mechanism of ArcS. Kinetic parameters of ArcS for lysine and tRNA-preQ<

SUBMITTER: Fujita S 

PROVIDER: S-EPMC11298593 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature