Systematic data analysis pipeline for quantitative morphological cell phenotyping.

ABSTRACT: Quantitative morphological phenotyping (QMP) is an image-based method used to capture morphological features at both the cellular and population level. Its interdisciplinary nature, spanning from data collection to result analysis and interpretation, can lead to uncertainties, particularly among those new to this actively growing field. High analytical specificity for a typical QMP is achieved through sophisticated approaches that can leverage subtle cellular morphological changes. Here, we outline a systematic workflow to refine the QMP methodology. For a practical review, we describe the main steps of a typical QMP; in each step, we discuss the available methods, their applications, advantages, and disadvantages, along with the R functions and packages for easy implementation. This revie

SUBMITTER: Ghanegolmohammadi F 

PROVIDER: S-EPMC11298594 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature