Caspases compromise SLU7 and UPF1 stability and NMD activity during hepatocarcinogenesis.

ABSTRACT:

Background & aims

The homeostasis of the cellular transcriptome depends on transcription and splicing mechanisms. Moreover, the fidelity of gene expression, essential to preserve cellular identity and function is secured by different quality control mechanisms including nonsense-mediated RNA decay (NMD). In this context, alternative splicing is coupled to NMD, and several alterations in these mechanisms leading to the accumulation of aberrant gene isoforms are known to be involved in human disease including cancer.

Methods

RNA sequencing, western blotting, qPCR and co-immunoprecipitation were performed in multiple silenced culture cell lines (replicates n ≥4), primary hepatocytes and samples of animal models (Jo2, APAP, Mdr2 ^-/- mice, n ≥3).

Results

SUBMITTER: Rojo C 

PROVIDER: S-EPMC11298840 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature