Adipose microenvironment promotes hypersialylation of ovarian cancer cells.

ABSTRACT:

Introduction

Ovarian and other peritoneal cancers have a strong tendency to metastasize into the surrounding adipose tissue. This study describes an effect of the adipose microenvironment on upregulation of sialic acid-containing glycans in ovarian cancer (OC). Heterogeneous populations of glycosylated OC tumors converged to a highly sialylated cell state that regulates tumorigenesis in an immune-dependent manner.

Methods

We modeled the adipose microenvironment by conditioning growth media with human patient-derived adipose tissue. OC cell lines grown in the presence vs. absence of adipose conditioned media (ACM) were characterized by transcriptomics, western blotting, and chemical biology glycan labeling methods. Fluorescence-activated cell sorting was used to separate adip

SUBMITTER: Fox A 

PROVIDER: S-EPMC11299042 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature