Bioprocessing of human platelet concentrates to generate lysates and extracellular vesicles for therapeutic applications.

ABSTRACT: This work describes protocols for preparing specific forms of human platelet lysates from pooled platelet concentrates (PCs) and the isolation of platelet-derived extracellular vesicles (p-EVs). Clinical-grade PCs can be sourced from blood establishments immediately following expiration for transfusion use. Here, we describe methods to process PCs into specific lysates from which p-EVs can be isolated. Each lysate type is prepared using platelet activation and processing methods which produce distinct products that may be useful in different applications. For example, serum-converted platelet lysate (SCPL)-EVs were recently shown to have powerful therapeutic properties following myocardial infarction in mice. EVs can be isolated from all products using size exclusion chromatography, produc

SUBMITTER: Yeh WT 

PROVIDER: S-EPMC11299553 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature