Combining array tomography with electron tomography provides insights into leakiness of the blood-brain barrier in mouse cortex.

ABSTRACT: Like other volume electron microscopy approaches, automated tape-collecting ultramicrotomy (ATUM) enables imaging of serial sections deposited on thick plastic tapes by scanning electron microscopy (SEM). ATUM is unique in enabling hierarchical imaging and thus efficient screening for target structures, as needed for correlative light and electron microscopy. However, SEM of sections on tape can only access the section surface, thereby limiting the axial resolution to the typical size of cellular vesicles with an order of magnitude lower than the acquired xy resolution. In contrast, serial-section electron tomography (ET), a transmission electron microscopy-based approach, yields isotropic voxels at full EM resolution, but requires deposition of sections on electron-stable thin and fragile

SUBMITTER: Kislinger G 

PROVIDER: S-EPMC11299977 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature