Genomic identification of expressed globulin storage proteins in oat.

ABSTRACT:

Introduction

Oats, a highly nutritious cereal known for their health benefits, contain various macromolecules of significant biological value, including abundant and highly digestible proteins. Despite their importance, oat proteins have not been extensively studied. Here, we present a complete set of the expressed globulins genes, which code for the main storage protein in oats as well as their chromosomal positions.

Methods

Published expressed sequence tags for globulins were used as queries in the Sang oat genome. In addition, globulin proteins were fractionated from oat flour by solvent extraction based on differential solubility with other classes of cereal proteins. The protein fractions were separated by gel electrophoresis and analyzed by tandem mass spectrometry to

SUBMITTER: Gil-Gonzalez AB 

PROVIDER: S-EPMC11300278 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature