Cholinergic stimulation stabilizes TRPM4 in the plasma membrane of cortical pyramidal neurons.

ABSTRACT: TRPM4 is a calcium activated non-selective cation channel, impermeable to Ca²⁺, in neurons it has been implicated in the regulation of the excitability and in the persistent firing. Cholinergic stimulation is also implicated in changes in excitability that leads neurons to an increased firing frequency, however it is not clear whether TRPM4 is involved in the cholinergic-induced increase in firing frequency. Here using a combination of patch clamp electrophysiology, Ca²⁺ imaging, immunofluorescence, fluorescence recovery after photobleaching (FRAP) and pharmacological approach, we demonstrate that carbachol (Cch) increases firing frequency, intracellular Ca²⁺ and that TRPM4 inhibition using 9-Ph and CBA reduces firing frequency and decreases the peak in int

SUBMITTER: Leyton P 

PROVIDER: S-EPMC11300349 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature