Barcoded overexpression screens in gut Bacteroidales identify genes with roles in carbon utilization and stress resistance.

ABSTRACT: A mechanistic understanding of host-microbe interactions in the gut microbiome is hindered by poorly annotated bacterial genomes. While functional genomics can generate large gene-to-phenotype datasets to accelerate functional discovery, their applications to study gut anaerobes have been limited. For instance, most gain-of-function screens of gut-derived genes have been performed in Escherichia coli and assayed in a small number of conditions. To address these challenges, we develop Barcoded Overexpression BActerial shotgun library sequencing (Boba-seq). We demonstrate the power of this approach by assaying genes from diverse gut Bacteroidales overexpressed in Bacteroides thetaiotaomicron. From hundreds of experiments, we identify new functions and phenotypes for 29 genes important for ca

SUBMITTER: Huang YY 

PROVIDER: S-EPMC11300592 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature