Establishment of a Transplantation Model of PDAC-Derived Liver Metastases.

ABSTRACT:

Background

The highly metastatic nature of pancreatic ductal adenocarcinoma (PDAC) and the difficulty to achieve favorable patient outcomes emphasize the need for novel therapeutic solutions. For preclinical evaluations, genetically engineered mouse models are often used to mimic human PDAC but frequently fail to replicate synchronous development and metastatic spread. This study aimed to develop a transplantation model to achieve synchronous and homogenous PDAC growth with controlled metastatic patterns in the liver.

Methods

To generate an orthotopic PDAC model, the DT6606 cell line was injected into the pancreas head of C57BL/6 mice, and their survival was monitored over time. To generate a heterotopic transplantation model, growing doses of three PDAC cell lines (DT6606,

SUBMITTER: Ferrara B 

PROVIDER: S-EPMC11300624 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature