Genetic modules for α-factor pheromone controlled growth regulation of Saccharomyces cerevisiae.

ABSTRACT: Saccharomyces cerevisiae is a commonly used microorganism in the biotechnological industry. For the industrial heterologous production of compounds, it is of great advantage to work with growth-controllable yeast strains. In our work, we utilized the natural pheromone system of S. cerevisiae and generated a set of different strains possessing an α-pheromone controllable growth behavior. Naturally, the α-factor pheromone is involved in communication between haploid S. cerevisiae cells. Perception of the pheromone initiates several cellular changes, enabling the cells to prepare for an upcoming mating event. We exploited this natural pheromone response system and developed two different plasmid-based modules, in which the target genes, MET15 and FAR1, are u

SUBMITTER: Gutbier U 

PROVIDER: S-EPMC11300815 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature