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Rhizoengineering with biofilm producing rhizobacteria ameliorates oxidative stress and enhances bioactive compounds in tomato under nitrogen-deficient field conditions.

ABSTRACT: Nitrogen (N) deficiency limits crop productivity. In this study, rhizoengineering with biofilm producing rhizobacteria (BPR) contributing to productivity, physiology, and bioactive contents in tomato was examined under N-deficient field conditions. Here, different BPR including Leclercia adecarboxylata ESK12, Enterobacter ludwigii ESK17, Glutamicibacter arilaitensis ESM4, E. cloacae ESM12, Bacillus subtilis ESM14, Pseudomonas putida ESM17 and Exiguobacterium acetylicum ESM24 were used for the rhizoengineering of tomato plants. Rhizoengineered plants showed significant increase in growth attributes (15.73%-150.13 %) compared to the control plants. However, production of hydrogen peroxide (21.49-59.38 %), electrolyte leakage (19.5-38.07 %) and malondialdehyde accumulation (36.27-46.31 %) were increased remarkably more in the control plants than the rhizoengineered plants, thus N deficiency induced the oxidative stress. Compared to the control, photosynthetic rate, leaf temperature, stomatal conductance, intrinsic and instantaneous water use efficiency, relative water content, proline and catalase activity were incredibly enhanced in the rhizoengineered plants, suggesting both non-enzymatic and enzymatic antioxidant systems might protect tomato plants from oxidative stress under N-deficient field conditions. Yield (10.24-66.21 %), lycopene (4.8-7.94 times), flavonoids (52.32-110.46 %), phenolics (9.79-23.5 %), antioxidant activity (34.09-86.36 %) and certain minerals were significantly increased in the tomatoes from rhizoengineered plants. The principal component analysis (PCA) revealed that tomato plants treated with BPR induced distinct profiles compared to the control. Among all the applied BPR strains, ESM4 and ESM14 performed better in terms of biomass production, while ESK12 and ESK17 showed better results for reducing oxidative stress and increasing bioactive compounds in tomato, respectively. Thus, rhizoengineering with BPR can be utilized to mitigate the oxidative damage and increase the productivity and bioactive compounds in tomato under N-deficient field conditions.

SUBMITTER: Haque MM

PROVIDER: S-EPMC11301190 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature

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Rhizoengineering with biofilm producing rhizobacteria ameliorates oxidative stress and enhances bioactive compounds in tomato under nitrogen-deficient field conditions.

Haque Md Manjurul MM Rupok Md Rahat Bari MRB Molla Md Abul Hossain MAH Rahman Md Mizanur MM Shozib Habibul Bari HB Mosharaf Md Khaled MK

Heliyon 20240708 14

Nitrogen (N) deficiency limits crop productivity. In this study, rhizoengineering with biofilm producing rhizobacteria (BPR) contributing to productivity, physiology, and bioactive contents in tomato was examined under N-deficient field conditions. Here, different BPR including Leclercia adecarboxylata ESK12, Enterobacter ludwigii ESK17, Glutamicibacter arilaitensis ESM4, E. cloacae ESM12, Bacillus subtilis ESM14, Pseudomonas putida ESM17 and Exiguoba ...[more]

PMID: 39108901

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Project description:Plant growth-promoting rhizobacteria (PGPR) not only enhance plant growth but also control phytopathogens and mitigate abiotic stresses, including water-deficit stress. In this study, 21 (26.9%) rhizobacterial strains isolated from drought-prone ecosystems of Bangladesh were able to form air-liquid (AL) biofilms in the glass test tubes containing salt-optimized broth plus glycerol (SOBG) medium. Based on 16S rRNA gene sequencing, Pseudomonas chlororaphis (ESR3 and ESR15), P. azotoformans ESR4, P. poae ESR6, P. fluorescens (ESR7 and ESR25), P. gessardii ESR9, P. cedrina (ESR12, ESR16, and ESR23), P. veronii (ESR13 and ESR21), P. parafulva ESB18, Stenotrophomonas maltophilia ESR20, Bacillus cereus (ESD3, ESD21, and ESB22), B. horikoshii ESD16, B. aryabhattai ESB6, B. megaterium ESB9, and Staphylococcus saprophyticus ESD8 were identified. Fourier transform infrared spectroscopy studies showed that the biofilm matrices contain proteins, polysaccharides, nucleic acids, and lipids. Congo red binding results indicated that these bacteria produced curli fimbriae and nanocellulose-rich polysaccharides. Expression of nanocellulose was also confirmed by Calcofluor binding assays and scanning electron microscopy. In vitro studies revealed that all these rhizobacterial strains expressed multiple plant growth-promoting traits including N2 fixation, production of indole-3-acetic acid, solubilization of nutrients (P, K, and Zn), and production of ammonia, siderophores, ACC deaminase, catalases, lipases, cellulases, and proteases. Several bacteria were also tolerant to multifarious stresses such as drought, high temperature, extreme pH, and salinity. Among these rhizobacteria, P. cedrina ESR12, P. chlororaphis ESR15, and B. cereus ESD3 impeded the growth of Xanthomonas campestris pv. campestris ATCC 33913, while P. chlororaphis ESR15 and B. cereus ESD21 prevented the progression of Ralstonia solanacearum ATCC® 11696TM. In a pot experiment, tomato plants inoculated with P. azotoformans ESR4, P. poae ESR6, P. gessardii ESR9, P. cedrina ESR12, P. chlororaphis ESR15, S. maltophilia ESR20, P. veronii ESR21, and B. aryabhattai ESB6 exhibited an increased plant growth compared to the non-inoculated plants under water deficit-stressed conditions. Accordingly, the bacterial-treated plants showed a higher antioxidant defense system and a fewer tissue damages than non-inoculated plants under water-limiting conditions. Therefore, biofilm-producing PGPR can be utilized as plant growth promoters, suppressors of plant pathogens, and alleviators of water-deficit stress.

Dataset Information

Rhizoengineering with biofilm producing rhizobacteria ameliorates oxidative stress and enhances bioactive compounds in tomato under nitrogen-deficient field conditions.

Publications

Rhizoengineering with biofilm producing rhizobacteria ameliorates oxidative stress and enhances bioactive compounds in tomato under nitrogen-deficient field conditions.

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