Amyloid-β peptide treatment reverses bone loss in the mandibular condyle via Wnt signalling pathway.

ABSTRACT:

Objective

To explore the effect of amyloid-β peptide (Aβ) on mandibular condyle to develop a new treatment for postmenopausal women with Temporomandibular joint osteoarthritis.

Methods

A murine bone loss model was established by ovariectomy. Microstructure parameters of the condyle were measured by microcomputed tomography before and after intraperitoneal injection with Aβ. Flow cytometry, Alizarin red staining, RT-qPCR assays, FITC/PI staining, Oil Red O staining and western blotting were used to evaluate the effect of Aβ on the osteogenic differentiation of mouse bone marrow stromal stem cells (mBMSCs).

Results

In vivo, condylar microstructure parameters increased. Serum osteoprotegerin and procollagen type 1 N propeptide increased in a dose-dependent manner after t

SUBMITTER: Chen Q 

PROVIDER: S-EPMC11301219 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature