A proposed pathway from D-glucose to D-arabinose in eukaryotes.

ABSTRACT: In eukaryotes, the D-enantiomer of arabinose (D-Ara) is an intermediate in the biosynthesis of D-erythroascorbate in yeast and fungi and in the biosynthesis of the nucleotide sugar GDP-α-D-arabinopyranose (GDP-D-Arap) and complex α-D-Arap-containing surface glycoconjugates in certain trypanosomatid parasites. Whereas the biosynthesis of D-Ara in prokaryotes is well understood, the route from D-glucose (D-Glc) to D-Ara in eukaryotes is unknown. In this paper, we study the conversion of D-Glc to D-Ara in the trypanosomatid Crithidia fasciculata using positionally labeled [¹³C]-D-Glc and [¹³C]-D-ribose ([¹³C]-D-Rib) precursors and a novel derivatization and gas chromatography-mass spectrometry procedure applied to a terminal metabolite, lipoarabinogalactan. Th

SUBMITTER: Iljazi E 

PROVIDER: S-EPMC11301363 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature