Multiomics Characterization of a Less Invasive Microfluidic-Based Cell Sorting Technique.

ABSTRACT: Fluorescence-activated cell sorting (FACS) is a specialized technique to isolate specific cell subpopulations with a high level of recovery and accuracy. However, the cell sorting procedure can impact the viability and metabolic state of cells. Here, we performed a comparative study and evaluated the impact of traditional high-pressure charged droplet-based and microfluidic chip-based sorting on the metabolic and phosphoproteomic profile of different cell types. While microfluidic chip-based sorted cells more closely resembled the unsorted control group for most cell types tested, the droplet-based sorted cells showed significant metabolic and phosphoproteomic alterations. In particular, greater changes in redox and energy status were present in cells sorted with the droplet-based cell sor

SUBMITTER: Choudhury FK 

PROVIDER: S-EPMC11301668 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature