Agreement between two real-time commercial PCR kits and an in-house real-time PCR for diagnosis of mucormycosis.

ABSTRACT: Mucormycosis is a severe and emerging invasive fungal infection associated with high mortality rates. Early diagnosis is crucial for initiating specific antifungal treatment, with molecular tools currently representing the most efficient diagnostic approach. Presently, a standardized in-house real-time PCR method is widely employed for diagnosing mucormycosis. Our study aimed to evaluate the agreement for the Mucorales DNA detection between two commercial real-time PCR assays-the Fungiplex Mucorales Real-Time PCR Kit and the MycoGENIE Aspergillus-Mucorales spp. Real-Time PCR Kit-in comparison with the in-house PCR. We retrospectively analyzed 58 samples previously identified as positive for Mucorales using the in-house PCR. These samples, obtained from 22 patients with proven or pro

SUBMITTER: Rafanomezantsoa LC 

PROVIDER: S-EPMC11302037 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature