Lactate dehydrogenase A (LDHA)-mediated lactate generation promotes pulmonary vascular remodeling in pulmonary hypertension.

ABSTRACT:

Background

High levels of lactate are positively associated with prognosis and mortality in pulmonary hypertension (PH). Lactate dehydrogenase A (LDHA) is a key enzyme for the production of lactate. This study is undertaken to investigate the role and molecular mechanisms of lactate and LDHA in PH.

Methods

Lactate levels were measured by a lactate assay kit. LDHA expression and localization were detected by western blot and Immunofluorescence. Proliferation and migration were determined by CCK8, western blot, EdU assay and scratch-wound assay. The right heart catheterization and right heart ultrasound were measured to evaluate cardiopulmonary function.

Results

In vitro, we found that lactate promoted proliferation and migration of pulmonary artery smooth muscle cells

SUBMITTER: Wu D 

PROVIDER: S-EPMC11302077 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature