Transplantation of miR-145a-5p modified M2 type microglia promotes the tissue repair of spinal cord injury in mice.

ABSTRACT:

Background

The traumatic spinal cord injury (SCI) can cause immediate multi-faceted function loss or paralysis. Microglia, as one of tissue resident macrophages, has been reported to play a critical role in regulating inflammation response during SCI processes. And transplantation with M2 microglia into SCI mice promotes recovery of motor function. However, the M2 microglia can be easily re-educated and changed their phenotype due to the stimuli of tissue microenvironment. This study aimed to find a way to maintain the function of M2 microglia, which could exert an anti-inflammatory and pro-repair role, and further promote the repair of spinal cord injury.

Methods

To establish a standard murine spinal cord clip compression model using Dumont tying forceps. Using FACS, to sor

SUBMITTER: Li P 

PROVIDER: S-EPMC11302162 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature