Engineering a dysbiotic biofilm model for testing root caries interventions through microbial modulation.

ABSTRACT:

Background

This study aimed to engineer and optimise a dysbiotic biofilm model to develop in vitro root caries for investigating microbial modulation strategies. The model involved growing complex biofilms from a saliva inoculum collected from four volunteers using two strategies. In the first strategy ("pre-treatment strategy"), bovine root slabs were used, and two natural compounds were incorporated at time 0 of the 10-day biofilm experiment, which included sucrose cycles mimicking the cariogenic environment. In the second strategy ("post-treatment strategy"), mature biofilms were grown in a modified Calgary biofilm device coated with collagen and hydroxyapatite for 7 days and then were exposed to the same natural compounds. The metatranscriptome of each biofilm was then determin

SUBMITTER: Dame-Teixeira N 

PROVIDER: S-EPMC11302357 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature