Efficiency of acetate-based isopropanol synthesis in Escherichia coli W is controlled by ATP demand.

ABSTRACT:

Background

Due to increasing ecological concerns, microbial production of biochemicals from sustainable carbon sources like acetate is rapidly gaining importance. However, to successfully establish large-scale production scenarios, a solid understanding of metabolic driving forces is required to inform bioprocess design. To generate such knowledge, we constructed isopropanol-producing Escherichia coli W strains.

Results

Based on strain screening and metabolic considerations, a 2-stage process was designed, incorporating a growth phase followed by a nitrogen-starvation phase. This process design yielded the highest isopropanol titers on acetate to date (13.3 g L^-1). Additionally, we performed shotgun and acetylated proteomics, and identified several stress conditio

SUBMITTER: Kutscha R 

PROVIDER: S-EPMC11302359 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature