Herpes simplex virus type 2 glycoprotein G-negative clinical isolates are generated by single frameshift mutations.

ABSTRACT: Herpes simplex virus (HSV) codes for several envelope glycoproteins, including glycoprotein G-2 (gG-2) of HSV type 2 (HSV-2), which are dispensable for replication in cell culture. However, clinical isolates which are deficient in such proteins occur rarely. We describe here five clinical HSV-2 isolates which were found to be unreactive to a panel of anti-gG-2 monoclonal antibodies and therefore considered phenotypically gG-2 negative. These isolates were further examined for expression of the secreted amino-terminal and cell-associated carboxy-terminal portions of gG-2 by immunoblotting and radioimmunoprecipitation. The gG-2 gene was completely inactivated in four isolates, with no expression of the two protein products. For one isolate a normally produced secreted portion and a truncated

SUBMITTER: Liljeqvist JA 

PROVIDER: S-EPMC113027 | biostudies-literature | 1999 Dec

REPOSITORIES: biostudies-literature