Absolute quantification of viral proteins from pseudotyped VSV-GP using UPLC-MRM.

ABSTRACT: The rapidly developing field of oncolytic virus (OV) therapy necessitates the development of new and improved analytical approaches for the characterization of the virus during production and development. Accurate monitoring and absolute quantification of viral proteins are crucial for OV product characterization and can facilitate the understanding of infection, immunogenicity, and development stages of viral replication. Targeted mass spectrometry methods like multiple reaction monitoring (MRM) offer a robust way to directly detect and quantify specific targeted proteins represented by surrogate peptides. We have leveraged the power of MRM by combining ultra-high performance liquid chromatography (UPLC) with a Sciex 6500 triple-stage quadrupole mass spectrometer to develop an assay that

SUBMITTER: Basu R 

PROVIDER: S-EPMC11302727 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature