A rapid LAMP assay for the diagnosis of oak wilt with the naked eye.

ABSTRACT:

Background

Oak wilt disease, caused by Bretziella fagacearum is a significant threat to oak (Quercus spp.) tree health in the United States and Eastern Canada. The disease may cause dramatic damage to natural and urban ecosystems without management. Early and accurate diagnosis followed by timely treatment increases the level of disease control success.

Results

A rapid assay based on loop mediated isothermal amplification (LAMP) was first developed with fluorescence detection of B. fagacearum after 30-minute reaction time. Six different primers were designed to specifically bind and amplify the pathogen's DNA. To simplify the use of this assay in the field, gold nanoparticles (AuNPs) were designed to bind to the DNA amplicon obtained from the LAMP reaction. Upon inducing pre

SUBMITTER: Novi VT 

PROVIDER: S-EPMC11302832 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature