MnoSR removal in Mycobacterium smegmatis triggers broad transcriptional response to 1,3-propanediol and glucose as sole carbon sources.

ABSTRACT:

Introduction

The two-component signal transduction systems play an essential role in the adaptation of bacteria to changing environmental conditions. One of them is the MnoSR system involved in the regulation of methylotrophic metabolism in M. smegmatis.

Methods

Mycobacterium smegmatis mutant strains ΔmnoS, ΔmnoR and ΔmnoS/R lacking functional mnoS, mnoR and both genes were generated using a homologous recombination approach. MnoR recombinant protein was purified by affinity column chromatography. The present study employs molecular biology techniques: cloning strategies, global RNA sequencing, qRT-PCR, EMSA, Microscale thermophoresis, and bioinformatics analysis.

Results and discussion

The ∆mnoS, ∆mnoR, and ∆mnoS/R mutant strains were generated and cultured in the pr

SUBMITTER: Plocinska R 

PROVIDER: S-EPMC11303327 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature