Rational selection of morphological phenotypic traits to extract essential similarities in chemical perturbation in the ergosterol pathway.

ABSTRACT: Terbinafine, fluconazole, and amorolfine inhibit fungal ergosterol synthesis by acting on their target enzymes at different steps in the synthetic pathway, causing the accumulation of various intermediates. We found that the effects of these three in- hibitors on yeast morphology were different. The number of morphological parameters commonly altered by these drugs was only approximately 6% of the total. Using a rational strategy to find commonly changed parameters,we focused on hidden essential similarities in the phenotypes possibly due to decreased ergosterol levels. This resulted in higher apparent morphological similarity. Improvements in morphological similarity were observed even when canonical correlation analysis was used to select biologically meaningful morphological parameters

SUBMITTER: Ghanegolmohammadi F 

PROVIDER: S-EPMC11303412 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature