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The cap-binding complex modulates ABA-responsive transcript splicing during germination in barley (Hordeum vulgare).


ABSTRACT: To decipher the molecular bases governing seed germination, this study presents the pivotal role of the cap-binding complex (CBC), comprising CBP20 and CBP80, in modulating the inhibitory effects of abscisic acid (ABA) in barley. Using both single and double barley mutants in genes encoding the CBC, we revealed that the double mutant hvcbp20.ab/hvcbp80.b displays ABA insensitivity, in stark contrast to the hypersensitivity observed in single mutants during germination. Our comprehensive transcriptome and metabolome analysis not only identified significant alterations in gene expression and splicing patterns but also underscored the regulatory nexus among CBC, ABA, and brassinosteroid (BR) signaling pathways.

SUBMITTER: Sybilska E 

PROVIDER: S-EPMC11303550 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature

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The cap-binding complex modulates ABA-responsive transcript splicing during germination in barley (Hordeum vulgare).

Sybilska Ewa E   Collin Anna A   Sadat Haddadi Bahareh B   Mur Luis A J LAJ   Beckmann Manfred M   Guo Wenbin W   Simpson Craig G CG   Daszkowska-Golec Agata A  

Scientific reports 20240807 1


To decipher the molecular bases governing seed germination, this study presents the pivotal role of the cap-binding complex (CBC), comprising CBP20 and CBP80, in modulating the inhibitory effects of abscisic acid (ABA) in barley. Using both single and double barley mutants in genes encoding the CBC, we revealed that the double mutant hvcbp20.ab/hvcbp80.b displays ABA insensitivity, in stark contrast to the hypersensitivity observed in single mutants during germination. Our comprehensive transcri  ...[more]

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