Temporal Patterns of Angular Displacement of Endosomes: Insights into Motor Protein Exchange Dynamics.

ABSTRACT: The material transport system, facilitated by motor proteins, plays a vital role in maintaining a non-equilibrium cellular state. However, understanding the temporal coordination of motor protein activity requires an advanced imaging technique capable of measuring 3D angular displacement in real-time. In this study, a Fourier transform-based plasmonic dark-field microscope has been developed using anisotropic nanoparticles, enabling the prolonged and simultaneous observation of endosomal lateral and rotational motion. A sequence of discontinuous 3D angular displacements has been observed during the pause and run phases of transport. Notably, a serially correlated temporal pattern in the intermittent rotational events has been demonstrated during the tug-of-war mechanism, indicating Markovi

SUBMITTER: Jin S 

PROVIDER: S-EPMC11304332 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature