Advancing preclinical drug evaluation through automated 3D imaging for high-throughput screening with kidney organoids.

ABSTRACT: High-throughput drug screening is crucial for advancing healthcare through drug discovery. However, a significant limitation arises from availablein vitromodels using conventional 2D cell culture, which lack the proper phenotypes and architectures observed in three-dimensional (3D) tissues. Recent advancements in stem cell biology have facilitated the generation of organoids-3D tissue constructs that mimic human organsin vitro. Kidney organoids, derived from human pluripotent stem cells, represent a significant breakthrough in disease representation. They encompass major kidney cell types organized within distinct nephron segments, surrounded by stroma and endothelial cells. This tissue allows for the assessment of structural alterations such as nephron loss, a characteristic

SUBMITTER: Oishi H 

PROVIDER: S-EPMC11304660 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature