Identification of Lytic Phages Against Multidrug-Resistant <i>Klebsiella pneumoniae</i>: Illuminating Hope on Antimicrobial-Resistance.

ABSTRACT:

Background

Lytic phages have been considered as a solution to mitigate the emergence of multidrug-resistant bacteria. Nevertheless, finding phages capable of targeting a broad host-range remains a significant challenge.

Materials and methods

Our study introduces two lytic phages isolated from hospital effluent, which are active against extended-spectrum cephalosporin-resistant Klebsiella pneumoniae.

Results

Overnight coculture with host, two purified phage lysates yielded around 3.0 × 10⁷ PFU/mL with an average 0.8 ± 0.2 mm diameter of clear, round, and non-halo plaques in both instances. The genomes of iPHaGe-KPN-11i (177,603 bp, 273 coding sequences [CDS]) and iPHaGe-KPN-12i (178,179 bp, 275 CDS) belong to the Pseudotevenvirus genus. Both ph

SUBMITTER: Rahman S 

PROVIDER: S-EPMC11304750 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature