Cxcl10 and Cxcr3 regulate self-renewal and differentiation of hematopoietic stem cells.

ABSTRACT:

Background

The function of hematopoietic stem cells (HSC) is regulated by HSC internal signaling pathways and their microenvironment. Chemokines and chemokine ligands play important roles in the regulation of HSC function. Yet, their functions in HSC are not fully understood.

Methods

We established Cxcr3 and Cxcl10 knockout mouse models (Cxcr3^-/- and Cxcl10^-/-) to analyze the roles of Cxcr3 or Cxcl10 in regulating HSC function. The cell cycle distribution of LT-HSC was assessed via flow cytometry. Cxcr3^-/- and Cxcl10^-/- stem/progenitor cells showed reduced self-renewal capacity as measured in serial transplantation assays. To study the effects of Cxcr3 or Cxcl10 deficient bone marrow microenvironment, we transplanted CD45.1 donor c

SUBMITTER: Liu F 

PROVIDER: S-EPMC11304843 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature